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Each formulation dilution was assessed in triplicate.
Each cell line was assessed in triplicate.
The stability of 111In-DTPA-11B6 was assessed in triplicate by incubating the compound at 4°C in PBS buffer or at 37°C in murine serum collected from normal NMRI mice.
Each sample was assessed in triplicate and analyzed in 3 independent experiments.
Each sample was assessed in triplicate and each experiment was performed at least 3 times.
For ELISA, 50 µL of supernatant were assessed in triplicate in 96-well ELISA microplates (Greiner Bio One).
Each experiment was run twice with a least 6 animals per group, and in both cases, samples were assessed in triplicate.
Schizont numbers were assessed in triplicate cultures by immunofluorescence assay using antibodies against PyHSP70.1 that recognizes P. yoelii liver stages as previously described [45].
For each reaction, 10 µL were assessed in triplicate on a 384-well plate and amplified using a LightCycler® 480 Real-Time PCR system (Roche).
Each reaction was run twice with a least 6 animals per group, and in both cases, samples were assessed in triplicate.
Final quantification was based on three independent experiments in which each treatment condition and RT primer setting were assessed in triplicate.
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