Behavioral impairments were assessed in the open field arena, in the Morris water maze and in the inhibitory avoidance task; volumetric extent of tissue damage was assessed using cresyl violet staining at adult age, after completing behavioral assessment.
Similarly none of the variables assessed in the open field test varied with treatment (Figure S3).
Locomotor activity assessed in the open field, is considered a well organized behavior in rodents determined by several factors, including anxiety, arousal, risk assessment, and exploration, and is an established tool for behavioral phenotyping in mice [24].
Animals were assessed in the open field, accelerating rotarod, light and dark preference, and nest-building tests as previously described (34, 39).
did not affect locomotor activity as evaluated in the open open-field test, and learning and memory when assessed in the inhibitory foot-shock avoidance task.
Hyperactivity in SHR has been assessed using the open-field method and the operant-conditioning method.
Locomotor activity was assessed by the open-field test following a protocol described previously [ 14].
Rat pups (n = 10 to 15 per group, 5 to 6 litters, 2 to 4 rats from each litter) were assessed for their behavior in the open-field and the elevated-plus maze between PND 29 and 33.
Animals received compound 9 via i.p. (30 mg/kg) route 30 min before testing, and the ambulatory behavior of the treated animals was assessed in an open-field test as described previously.
