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The outcome was assessed in each case.
Also, the percentage of nuclei was assessed in each case and scored on a scale of 0 to 100%.
For each analysis, the residuals from the fitted model were graphically assessed; in each case the standard assumptions of normality, constant variance and randomness were confirmed.
The presence of subchondral bone marrow replacement by fibrovascular tissue was assessed in each case as either present (pathologic) or absent (normal).
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At least three biopsy fragments were assessed in each cases.
To assess in each case whether lethality involved inhibition of system xc− and induction of ferroptosis, as opposed to the induction of another form of death, experiments were performed ±β-ME.
The suitability of such manoeuvres needs to be assessed in each individual case and will also depend on availability of an interventional radiologist.
For these reasons, the quality assurance system needs to be capable of evaluating the quality metric on prescribed ROIs, as well as the entire image, an report the assessed quality in each case.
We note, however, that toxicity could be greater at higher RNP concentrations or with RNPs targeted to different sites and will need to be assessed carefully in each case.
For each patient, slides prepared using both techniques were compared and assessed and in each case an attempt was made to analyse the reasons for any potential differences.
However, their accuracy and spatial resolution need to be assessed in the case of III-nitrides.
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