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Growth inhibition was assessed from the changes in total cellular protein after 48 hours of drug treatment using a sulphorhodamine B assay.
Insulin secretion in response to glucose ramping was assessed from the changes in C-peptide concentrations and the prehepatic insulin secretion rate (ISR).
The primary outcomes were (1) a shift from sedation-based to analgesia-based sedation (analgo-sedation), assessed from the changes in the use of sedatives and analgesic agents in each patient, and (2) improved pain management, assessed daily in each patient using the Pain Intensity scale [ 21].
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The stability of mixed micelles in bovine serum albumin (BSA) solution was assessed from the change of particle size of micelles and the micellar propofol upon incubation of the mixed micelles with 0.2% BSA solution [24, 25].
Clinical outcomes were assessed from the absolute change in hospitalized days, total number of psychotropic medications prescribed, and historic Clinical Global Impression severity scores.
Finally, the actual impact at the regimes needs to be assessed from the bottom-up perspective of changes in social practice and potential regime reactions and alignment.
DDE displays anti-androgenic effects in vivo, as assessed from changes in the weights of androgen-responsive tissues (Owens et al. 2007).
Changes in the plant diversity indicator were assessed from effects of changes in the occurrence of target and competing plant species in response to changes in mean spring groundwater level and in pH and NO3 concentration, as calculated with SMART2.
Tumour response was assessed from relative change in the tumour volume in relation to the volume at the beginning of therapy.
Therapy response can be assessed from changes in both these metrics or from a change in the total lesion glycolysis that is calculated as the product of the average SUV in the tumour and the volume [23].
Changes in anthropogenic pressure on land were assessed from changes in population and livestock.
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