Telomere length was assessed from cells isolated from saliva using quantitative polymerase chain reaction methods.
Basal NFAT localization was assessed from cells before store depletion.
The proportion of activated NK cells was assessed from the cells expressing CD69, an early activation marker of lymphocytes [ 18], within the CD56+CD3- NK cell fraction.
These results indicate that microbes can induce specific signatures of immunoglobulin gene rearrangements and that pathogen exposure can potentially be assessed from B cell repertoires.
The presence of active downstream/executioner caspases viz caspase3/7, was significantly reduced in the test sets as assessed from a cell free cytosolic enzymatic assay (Figure 1B, p 0.010, p 0.017, n = 4).
Type II collagen (CII -specific CII -specificoliferative response and CD4okine production were assessed from lymph node cell culture.
Expression of Tgfβ isoforms and receptors was assessed from untreated control cells.
Other limitations were the lack of quantification of ischemic myocardium as a result of the I/R, and that we used whole left ventricular tissue rather than isolated cardiomyocyte digestion, and were thus not able to assess from which cell type the vasopressin receptors were derived.
Cx26 expression was assessed from Western blots of cell lysates using antibodies against the histidine tag (anti-Penta-His, Qiagen) or the Cx26 intracellular loop region (Life Technologies).
The biocompatibility of the scaffolds was assessed from the response of cells to extracts of the dissolution products of the scaffolds, using assays of MTT hydrolysis, cell viability, and alkaline phosphatase activity.
