Draft genome bins were then assessed for quality, contamination, and completeness using CheckM v.1.0.566.
Short sequencing reads were extracted using fastq-dump and assessed for quality using fastQC (see URLs).
RNA was assessed for quality and was quantified using RNA 6000 Nano LabChip on a 2100 Bioanalyzer (Agilent).
Total RNA was assessed for quality using an Agilent Tapestation, and all samples had RNA Integrity Numbers above 9.0.
The articles were assessed for quality using the MMAT and AACODS checklist.
Data from 27 studies were extracted and assessed for quality using standardized tools.
Studies were assessed for quality, analysed descriptively and meta-analysed when possible.
Randomized controlled trials were assessed for quality of evidence using the GRADE system.
Following extraction, RNA was assessed for quality by visualisation on a 1.2% agarose gel, and quantified using a Nanodrop spectrophotometer (Thermo scientific).
RNA was quantified spectrophotometrically (Nanodrop, Thermo Scientific, Wilmington, DE) and assessed for quality by capillary gel electrophoresis (Agilent 2100 Bioanalyzer; Agilent Technologies, Inc., Palo Alto, CA).
Each study included in this review was assessed for quality as good, moderate, or poor based on biases using the modified Hayden's criteria30.
