In addition, we assessed expression of MtSCP1, and MtBCP1, two genes expressed in this cell type that are suitable as additional markers.
Since the most important TGFβ isoform in humans is represented by TGFβ1, we assessed expression of this particular molecule in our study.
Therefore, we assessed expression of M2 macrophage-associated genes in chitin-elicited PECs and found that expression of genes encoding Arg1 and MR was significantly lower in PECs isolated from Rbpj cKO mice compared with WT controls (Fig. 2A).
We then assessed expression of IL-1R1, IL-R2 and TNFR2 on these activated subsets.
We assessed expression of other AAM-associated markers in the CD11c+ cells.
We assessed expression of XRCC3 and KU80 proteins on 60% confluent cultures by Western blot of nuclear and cytoplasmic fractions.
As a measure of Hedgehog pathway activity in the various mouse models, we assessed expression of total Gli2 transcripts using total RNA from tibias and cultured osteoblasts.
In addition, we only assessed expression of insulin signalling proteins in the basal state – i.e. signalling protein expression in response to fasting insulin concentrations – and in response to maximal insulin stimulation.
In one study comparing gene expression in different tissues, Glatt et al [31] assessed expression of prefrontal cortex (PFC) post-mortem brain and peripheral blood cells from different cohorts of SZ patients and controls, in order to identify genes with differential expression across populations and tissue types.
To validate this list and our estimates of gene expression level from the microarrays we assessed expression of 24 of the 26 genes in the final cluster to gata3 by real time RT-PCR in the cell line VOT-E36 after 2 days of differentiation in vitro (Table 4).
I next assessed expression of cyclinG1, p53 target gene, after mRNA injections by visually scoring expression strength as "high" or "low" based on staining of uninjected embryos (Fig. 6B), where embryos with "high" cyclinG1 expression were inferred to be shh−/− mutant and those with "low" expression were wild-type (control stainings of wild-type embryos, not shown).
