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We assessed expression levels of GADD34, BiP and GRP94.
We also assessed expression levels of the autophagy-related genes Atg7, Atg8a and Atg12.
In this study, we first assessed expression levels of miR-133a in 34 normal, 26 benign, and 90 cancerous tissue samples using in situ hybridization.
Next, we assessed expression levels of the components of the LIF signal transduction pathways summarized in Fig. 3A by quantitative PCR (qPCR) in ESCs cultured in 2iLIF.
Moreover, we extracted RNA from MCF10A cells expressing an empty vector, HER2, HER3 or HER2/HER3 that were grown in three-dimensional cultures for 15 days and assessed expression levels of epithelial-to-mesenchymal transition (EMT) markers.
In a first attempt to provide insight into possible mechanisms underlying the enhancement of hippocampal precursor cell proliferation by Th17 cell-secreted cytokines, we assessed expression levels of mRNAs encoding relevant cytokine receptors in the neurogenic niche.
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Quantitative RT-PCR was performed to assess expression levels of responsive genes under normal or drought and salt stress.
Erythroid development was determined by assessing expression levels of transferrin receptor (CD71) and glycophorin A (CD235a) by flow cytometry.
To assess expression levels of dystrophin-associated glycoproteins (DAG), KCl-washed microsomes were prepared from wt, mdx, and ms-DKO mice for western blot analysis.
qPCR has extensively been used to assess expression levels of both protein coding genes and miRNAs in C. elegans.
Respondents were asked about the use of non array-based approach (e.g., real-time PCR) to assess expressionon array-basedcific genes.
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