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To test this hypothesis further, we briefly incubated tendons in trypsin, embedded the tendons in fibrin gels, and assessed cell migration after 5 days.
To understand how adhesions form we incubated excised tendons in fibrin gels (to mimic the homeostatic environment at the injury site) and assessed cell migration.
For this, we assessed cell migration using the Transwell assay, as previously described [ 12].
There are few in vivo meniscal repair studies that have assessed cell migration and proliferation and extracellular matrix deposition.
We assessed cell migration by determining the ability of the cells to move into an acellular space in a two-dimensional in vitro wound healing assay.
We assessed cell migration by determining the ability of the cells to move into an acellular space in a two-dimensional in vitro "wound healing assay" [ 29].
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Images were analysed using Image J to assess cell migration.
To assess cell migration, the speed and directionality of cells were measured using the Manual Tracking plug-in of ImageJ.
The Boyden chamber assay provides a convenient method of assessing cell migration and measuring cell motility coefficients at the population level.
To assess cell migration rates, corneas were subjected to 1.5 mm wounds and allowed to heal for 12, 15, 18, 21, and 24 h.
As future pre-clinical applications may include lung evaluation for MSCs infusion in repair processes, such as broncho-pleural fistulae [23], and assessing cell migration into the lungs, we also labelled MSCs with PFC nanoemulsions, which can be detected with 19F MRI and MRS [9, 10].
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