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As shown in Figure 10 A, approximately 6000 CD4+ cells (assessed by counting of CD4+ in a Bürker-Türk chamber) [28] were migrated (after 10 h incubation) from the upper chamber towards the untreated human adipocytes (lower chamber), indicating that adipocyte-associated chemokines are functional.
We saw no significant effect of the tcf7l2 mutation on the asymmetric production of HuC/D-positive or HuC GFP-transgene-positive neurons, as assessed by counting of cells at 34, 36, 39, and 48 HuC GFP-transgene-positiveterality < 0.001, page < 0.001, no significant first-order interactions, ANOVA; Figures 3B–3F and Table S2).
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Study drug compliance (assessed by count of capsules returned by subject) and lifestyle counseling were addressed at each study visit, conducted every 4 weeks.
Cell growth was assessed by counting number of viable cells using the Vi-cell automatic counter (Beckman).
The relationship between intervention measures (e.g., culling and impounding of free-roaming dogs) and the time series of cases was assessed by counting the number of cases before and after implementation of these control measures.
Thus the mean numbers of positive cells (± standard error) per field of view were obtained; the total number of cells sampled per field of view was assessed by counting the numbers of nuclei stained by DAPI.
The data are mean numbers of positive cells (± standard error) per field of view; n = mean number of total cells sampled per field of view as assessed by counting the numbers of nuclei stained by DAPI.
Whole mounts were used to calculate total area of the fat pad and the degree of branching morphogenesis (assessed by counting the numbers of primary, secondary and tertiary branch points).
Prevalence was assessed by counting the number of reefs affected and the number of reef-years of impact for each agent.
In brief, the degree of inflammation was assessed by counting the number of cellular infiltrates in the spinal cord.
Thus, the effect of treatment was assessed by counting the number of viable Salmonella not only in the gut (cecum) but also in the liver and spleen.
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