Sentence examples for assessed binding to from inspiring English sources
To address if QIL1 also interacted with MIC10, we immunopurified endogenous MIC10 in isolated mitochondria from 293T cells and assessed binding to QIL1 by western blot analysis.
To assess binding to the native conformation, conditions that readily denature myosin, e.g. dilute protein concentrations at elevated temperatures, were avoided.
However, we wished to assess binding to combinations of sphingolipids and gangliosides in a more membrane-like setting that would support microdomain formation.
Accordingly we employed recombinant BSP, BSP purified from bone, or recombinant human BSP to assess binding to proMMP-2.
Radioligand binding assays were contracted to CEREP (Celle l'Evescault, France) where membranes from CHO-cells expressing either hCB1R or hCB2R were used to assess binding to the two cannabinoid receptors.
A direct interaction was next explored by assessing binding to the ENTH domain of epsin 1 (with an His tag at the C-terminus) to increasing concentrations of the cytosolic portion of Syb2 fused to GST.
To validate the binding of NLX to FLNA, we assessed binding of [3H]NLX to membranes prepared from the human melanoma cell line M2 that lacks filamin and to membranes from its FLNA-transfected subclone A7.
Since TESS predicted that Sp1 binds to MCRE, we assessed binding of cellular Sp1 to MCRE by electrophoretic mobility shift assay (EMSA).
Two mechanisms were assessed: binding of the tracer to melanin or to sigma receptors of melanoma cells.
We assessed binding of serum IgG to 3 in the absence of a competitor, or in the presence of 500 nM insulin or GAD65.
