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We assessed assay reproducibility as measured by the intraclass correlation coefficient (ICC) and coefficient of variation among seropositives (CV) for GST-L1, cLIA, and SEAP-NA using 25 blind duplicate specimens included in each batch.
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To assess assay performance and diagnostic specificity, 192 bovine respiratory samples were analyzed by incorporating a high throughput DNA extraction platform.
To assess assay precision, we studied the intra-assay and inter-assay variability of each technique for GAPDH and PMLC20.
Having replicated compounds in the compound library was useful for assessing assay reproducibility.
To assess assay reproducibility we measured the dose-dependent unwinding induced by X-irradiation of cells in several independent experiments.
Multiple identical control samples were assigned to each batch to assess assay variability and control batch effects.
Dilution series of patient DNA diluted into wild-type DNA (from peripheral blood cells) was used to assess assay sensitivity each time the assay was performed.
We next assessed the assay in a reporter gene format.
The formulations were assessed on assay, dissolution, friability, weight variation and disintegration time.
Biomechanical changes were assessed by assaying RBC membrane deformability (RBCd) using the micropipet aspiration technique.
In vitro significance of ERK5 signalling was assessed by assays for proliferation, motility, invasion and invadopodia.
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