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To identify the key functional pathways downstream of activated JAK2, we assessed activation of Stat1, 3, and 5, Pi3k/Akt, and Mek/Erk in the presence and absence of TG101209.
To confirm apoptosis, we assessed activation of cellular caspase 3 using FITC-conjugated active caspase 3 antibody, followed by FACS analysis.
We injected insulin into fasted mice and assessed activation of the insulin signaling pathway by Western blot (Fig. 4).
As PI3K under certain conditions can interact with the MAPK/ERK pathway, we also assessed activation of phospho-ERK.
Moreover, we assessed activation of BAX as a marker of mitochondrial apoptosis.
We also assessed activation of caspases in the absence or presence of zVAD-fmk.
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A specific caspase-inhibitor CaspACE FITC VAD FMK In Situ Marker (Promega, Mannheim, Germany) was used to assess activation of Caspase-like cysteine protease activity in the parasite, following manufacturer's instructions.
Scintigraphy using 99mTc-3PRGD2 targetintegringrin αvβ3 could assess activation of hepatic stellate cells (HSCs).
Many questions remain regarding the use of TSPO tracers as diagnostic tools to assess activation of microglia.
The morphological change of the adherent platelets is a common qualitative criterion to assess activation of adherent platelet on the materials' surface.
To assess activation of muscles of hip adduction using EMG and force analysis during standard clinical tests, and compare athletes with and without a prior history of groin pain.
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