We assessed the replication of the seven lead single nucleotide polymorphisms (SNPs) and evaluated these loci for additional signals in American Indians.
To assess the replication capability of the VP6 mutant viruses in normal BSR cells, 10 μl of VP6-truncated BTV recovered from transfected BSR-VP6 cells was inoculated into normal BSR cells.
While qPCR has been utilized to assess the replication kinetics of R. felis in vitro [12], in vivo (arthropod and vertebrate) models of infection, for the purpose of examining the infection kinetics of R. felis, have not been examined.
Primer extension and steady-state kinetic studies using pol IV, exo-free Klenow fragment (KF (exo–)), and Dpo4 were performed to further assess the replication efficiency and fidelity of S-cdA and S-cdG.
As an initial indicator of the chromatin state, we assessed the replication timing of a cohort of genes in each cell type, based on data that expressed genes and acetylated chromatin domains, generally, replicate early in S-phase, whereas some silent genes, hypoacetylated or condensed chromatin tend to replicate later.
In order to directly compare the epigenetic profiles of extra-embryonic stem cell lines with those of pluripotent cell lines, we initially assessed the replication timing of a panel of developmental genes in OS25, B1 and IM8A1 cell lines.
We have subsequently designed and assessed the replication phenotypes and fitness of mutants of E7 constructed in such a way that allows effects of codon pair and dinucleotide frequency alterations to be separately altered.
The dimensions of the macroscopic features of the positive polymer and NaCl templates were compared to assess the accuracy of replication.
In particular, tests of measurement invariance were conducted across the present sample and the original sample from Maïano et al. (2009) study in order to more precisely assess the degree of replication of the results.
To assess the influence of replication on substitution rates in transcribed regions, we restricted our analysis to the set of human genes located within 1 of the 678 "N-domains" identified by Huvet et al. (2007), where start and stop positions of an "N-domain" give putative locations of two adjacent ORIs.
