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A pBluescript SK vector carrying a Homo sapiens desmin cDNA (accession N° BC032116) was added at 1,000 copies to the initial library in order to assess the normalization efficiency.
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Unfortunately we didn't realize a second lumbar puncture to assess the pleocytosis normalization during the days following the first lumbar puncture.
To assess the efficacy of normalization, the bitter melon sequences from both non-normalized and normalized libraries with Arabidopsis homologs were counted to be 6,737 and 2,187, respectively (Table 1).
Prior to normalization pin-tip (block) intensity box plots were used to detect slides exhibiting spatial and/or pin biases, with post-normalization intensity box plots and MA plots used to assess the effectiveness of normalization in correcting these biases.
To assess the effect of normalization on the outcome of the different packages, we considered both the use of the default normalization of each package, as well as the use of similarly preprocessed data normalized with the TMM normalization [ 18].
To assess the effect of normalization on variation for data in Figure 7 we calculated IQR for M values of identified populations.
The development of good practice for designing and administering structured instruments to assess the processes of normalization described and explicated in the formal specification of the theory is the next step for further extending the utility of NPT.
Spike-in probe intensities were used to assess the performance of the normalization procedure for each dataset.
To assess the efficiency of the normalization process for metatranscriptome samples, we constructed two cDNA libraries, one normalized and the other non-normalized.
In addition, three datasets with suitable positive controls were used to assess the impact of the normalization approaches on the power to identify significant enrichment for specific genomic regions.
To assess the relationship between the normalization of oscillatory activity in different brain areas and the reduction of tinnitus severity, we used the partial least-squares (PLS) multivariate approach that holds specific advantages over conventional univariate approaches [McIntosh et al., 1996].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com