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To assess the localization of FoxO1, we performed immunofluorescence staining using a specific FoxO1 antibody in NDPK-B depleted and control ECs.
Unfortunately, the methodology used to assess the localization of the SPG was not described nor was the size or demographics of the sample defined.
We sought to assess the localization of endogenous Rnd3 and Syx in adherent cells (which give better spatial resolution of the cytosolic compartment than ES cells).
The objective of present study was to further assess the localization of MTA1 in mouse epididymis on both transcriptional and translational level, and then to explore whether MTA1 expression is regulated by androgens and postnatal epididymal development.
In fact, a number of clear examples of incomplete detergent solubilization of known leptospiral outer membrane proteins, including the porin, OmpL1, have been described [22], [45], [47], [53], indicating that complete fractionation into the Triton X-114 detergent phase may not occur for transmembrane OMPs and that additional methods are needed to assess the localization of leptospiral proteins.
We were unable to assess the localization of IMC13 in the MORN1-KO background since expression of YFP-tagged IMC13 was only achieved with the Tet-on system [4], which is not compatible with the Tet-off system controlling Myc2-MORN1.
Similar(41)
We assessed the localization of pTyr176-AKT when Ack1 was activated.
To mechanistically link changes in the H3K4me3 patterns, we assessed the localization of ATX1 at the WRKY70 promoter using the ChIP assay with anti-ATX1 antibodies.
Since a lack of stable interactions between K-MTs was observed in the above experiments, we assessed the localization of BubR1 in oocytes from the ERK3-deleted group.
To begin to explore these mechanisms, we assessed the localization of markers of apical and basolateral polarity in flies with mutations in Dcas and Fak56.
We assessed the localization of L22 proteins truncated at either the N- or C- terminus and found that truncation did not alter the localization of L22 (Fig. 6A: Δ1–9 and Δ120 128; data not shown).
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