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To assess the functional implications of the association between PI3K-pathway alteration and MYC amplification in human prostate tumors, we turned to genetically engineered mouse (GEM) models.
To assess the functional implications of relapse, employment status (employed or otherwise) was reported at baseline and follow-up.
To assess the functional implications of recombination events between paralogs, we mimicked recombination by constructing several chimaeric FLO genes, each combining the 5′ end of one FLO gene with the 3′ end of another (supplementary Table S3 online).
Future plans include increased integration of additional annotation data that assess the functional implications of the variation, including but not limited to intersection of SNP locations with known or predicted functional elements such as splice sites, promoter elements, and polyadenylation sites or overlap with regions of evolutionary conservation, such as provided by the PhastCons scores (21).
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Further studies have to be performed to elucidate this phenomenon in detail and to assess the functional implication of these results.
DASH score was used to assess the functional outcome.
On the other hand, experimental assessment of the functional implications of all identified mutations is impractical.
We evaluate the adaptive evolution of 12 protein-coding mitochondrial genes in 41 placental mammalian species by assessing amino acid sequence variation and exploring the functional implications of observed variation in secondary and tertiary protein structures.
The functional implications of this amplification are currently being assessed.
The functional implications of a larger cortical representation can be assessed by means of psychophysical methods.
To better understand the functional implications of these mutations, a variety of biochemical and cellular assays, as well as computational methods, have been developed to assess the impact of the individual mutations.
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