We used real-time quantitative PCR to assess the expression change of these 52 genes, and defined these results as our "gold standard".
Using antibodies specific to mammalian mitogen-activated protein kinases (MAPK) and PKCs in a microarray approach, we assessed the expression changes of promastigotes proteins associated with macrophage attachment.
To investigate the possible role of BMP-2/Runx2 or Wnt/β-catenin signaling in osteoblast differentiation of AECs, we assessed the expression change of BMP-2, Runx2 and β-catenin mRNA in AECs with different treatments.
A second ERα-responsive cell line, the MCF-7 line, was also used to assess whether the expression changes could be observed in an independent genotype.
To assess how the expression changes were reflected in the protein level and distribution, we raised a rabbit polyclonal antibody using a synthetic peptide (GPYQSGRFYTKPPQC) corresponding to amino acids 43 57 of human sFRP1.
In addition, in this experiment we had time-matched naïve control samples at each time point, so we could assess the gene expression changes over time in the control animals, or basal expression variation.
To quantitatively assess the gene expression changes brought about by the absence of Insm1, we performed RNA-Seq using FACS-purified progenitor cells isolated at E15.5.
Because many microarray datasets have only a small number of samples, we applied a gene randomizing gene set analysis method [ 25, 26] to assess the overall expression changes in each gene set.
By using transcriptome profiling, we assessed the gene expression changes of the common subset of CBP, RA and 5-aza-dC targets upon overexpression of human TDG in HEK293 cells.
Taylor et al. [27] assessed the gene expression changes after TAM treatment in an ER+ estrogen-responsive breast cancer xenograft model to predict endocrine sensitivity.
