Sentence examples for assess sphere from inspiring English sources

Thus, this assay can assess sphere proliferation.

To assess sphere forming capacity, cells were trypsinized and plated in sphere media (DMEM-F12, 0.4% BSA (Sigma), 10 ml/500 ml B27 (Invitrogen) 5 μg/ml bovine insulin (Sigma), 4 μg/ml heparin (Sigma), 20 ng/ml fibroblast growth factor 2 (bFGF, Sigma) and 20 ng/ml epidermal growth factor (EGF, Sigma)) into 96-well ultra-low adhesion plates, ranging from 1 to 256 cells/well.

To assess sphere-forming efficiency after estrogen treatment or endocrinolectomy, pituitaries were harvested 3 and 6 days after surgery, respectively, and spheres were counted blindly and manually after 1 week in culture.

To assess sphere-forming ability in serum and non-serum containing media in nonadherent conditions, single FACS-sorted CD133+ or CD133- cells were seeded into low attachment 96-well plates.

Adult SVZ NS were sorted into a 96-well plate containing medium at 20 cells per well and allowed to grow for 2 weeks before assessing sphere formation.

Considering that cell aggregation might confuse true sphere formation in liquid culture, we further assessed sphere formation in methylcellulose-containing semisolid culture to rule out this confusion.

We then seeded one cell from each subpopulation in individual wells of a 96-well plate and assessed sphere formation after 14 days.

But what's most important for all of this, in my mind, is to accurately assess your sphere of influence and work within that sphere of influence.

MCF7 cells were transiently transfected with CSCG-PEA15 constructs using Lipofectamine-2000 and seeded in methylcellulose to assess for sphere formation.

DOI: http://dx.doi.org/10.7554/eLife.00940.009 To assess whether sphere growth was achieved by cell proliferation or by other mechanisms like cell migration and aggregation, we analyzed spheres by immunostaining and time-lapse imaging.

Previously, scattering technology has been applied to assess micron spheres on silicon wafers [ 16]; it can also be applied to identify collected microbial contaminants from different food sources [ 17].