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Further work is required to refine this approach and rigorously assess its analytical and diagnostic specificity and sensitivity.
To assess rigorously the potential under-annotation of short ORFs with homology, we exhaustively compared the polyORFome all possible ORFs in 64 prokaryotes (53 bacteria and 11 archaea) plus budding yeast to itself and to all known proteins.
The researchers will assess the results rigorously and thus gain confidence as to whether or not they are qualified.
Given the small sample size at each participating SPH we could not assess rigorously the validity or reliability of the tool, but future studies with larger samples could usefully contribute to this.
This is particularly difficult when schools are being assessed very rigorously on how many A*-C students they have.
However, study quality within each of the levels of evidence needs to be assessed more rigorously.
We put considerable effort into identifying trials that appeared to report mortality, but we have no way of assessing how rigorously this was done.
Accordingly, if noninferiority trials are to be applied to clinical and regulatory decisions regarding the marketing and use of new treatments, the assumptions must be made explicit and their influence on the resultant conclusions must be assessed rigorously.
At present, the usefulness and predictive ability of modeling and reverse engineering cannot be assessed and compared rigorously.
The effect of model uncertainty could be assessed rigorously since all bias and variance estimates were derived for specific models and for different test and training partitions.
Since KIEs may impact isotopic data and ultimately jeopardize the validity of the biological interpretations, their impact must be assessed rigorously.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com