The selected uncompleted buildings were assessed for surface salt deposits, crack formations and reinforcement corrosion.
After pre-treatment, the state of the sample surfaces was assessed for surface phases with grazing incidence X-ray diffraction (GIXRD), and XPS (X-ray photoelectron spectroscopy), evaluated by atomic force microscopy (AFM) and wettability, using the sessile drop test.
Human peripheral blood monocytes were assessed for surface expression of TLRs 1, 2, and 4.
Cells were assessed for surface marker expression using fluorescent multicolor flow cytometry (FACSCanto II; Becton Dickinson, San Jose, CA, USA) as described previously.
Where indicated, cells were pronase treated to remove preexisting surface coreceptors and placed in complete medium and assessed for surface coreceptor re-expression after 16 18 h (Suzuki et al, 1995).
Neutrophils were assessed for surface expression of A2MG receptor (LRP1) along with the lineage specific lineage marker (CD11b) under resting conditions and after LPS incubation (1 μg/ml, 1 h, 37°C).
Non-permeabilized cells were incubated on ice and incubated with an anti-GFP antibody (detected with a secondary antibody labeled with Alexa-546) to assess for cell surface exposure of ss-GFP-FM4-CD8.
The differences between first and second occasion measurements were assessed for each surface type by measurement of tooth surface area, hypoplastic lesion and opacity.
Films composed of collagen or gelatin and crosslinked with a carbodiimide were assessed for their surface roughness and stiffness.
To assess for changes in CD22 surface expression in the NHL xenografts after treatment, fine needle aspirations (FNA) were done serially; the samples were examined by flow cytometry for CD22 surface expression.
