Sentence examples for assess expression in from inspiring English sources
To assess expression in living humans, these studies have been extended to the assessment of gene expression via analysis of mRNA from circulating mononuclear cells in whole blood [ 75, 76].
To assess expression in eyes, eyes were pooled and homogenized in phosphate buffered saline after which Triton X-100 was added to a final concentration of 1%.
Western blot was used to assess expression in different tissues using the raised rabbit anti-5'Nuc polyclonal serum (1/1000) and a peroxidase-conjugated anti-rabbit IgG (1/1000, Sigma).
The mixture was extracted 1 2 hours with gentle agitation after which insoluble material was removed by centrifugation at 19,000× g for 30 min. To assess expression in brain, post-nuclear supernatants of brain were made by homogenizing brains in 20 mM Hepes pH 7.5, 250 mM sucrose, 2 mM MgCl2 and centrifuging at 1300× g for 5 minutes.
After construction, pVAP38 was first electroporated into M. smegmatis strain mc155 to assess expression in a test system.
The whole-mount lacZ reporter gene workflow presented in Fig. S2A was used to assess expression in tissues, as highlighted in Fig. 1 (see also Table S1).
Among a panel of novel and/or differentially methylated loci that we identified, we further characterized HOXD3 and BMP7 using a combination of MassARRAY® EpiTYPER analysis and quantitative MethyLight assay, and assessed expression in DU-145 PCa cells.
To determine the clinical relevance of the targets identified in the RNAi screen, we assessed expression in two patient series.
For assessing expression in vivo, the uninfected comparator consisted of cDNA synthesized from pooled purified RNA extracted from biopsies of 10 randomly selected H. pylori-negative patients.
The present study was designed to assess expression of iNOS in AAA in human beings.
