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Fluorescein diacetate (FDA) was used to visually assess cell viability (Widholm 1972).
HEK 293T cell was selected in the present study to assess cell viability and spreading on aligned CNF.
Assay methodologies vary widely (e.g. measurements of mitochondrial activity, ATP concentrations, and membrane integrity) but the basic principle is to assess cell viability and/or proliferation.
After incubation, the Neutral Red Uptake (NRU) assay was performed to assess cell viability in each insert as previously described, based on the National Institutes of Health [28].
Wild type HIV and envelope-minus HIV pseudotyped with Vesicular Stomatitis Virus glycoprotein (VSVg) were used as controls to assess cell viability after inhibiting clathrin pathway.
Positron emission tomography (PET) imaging was used to assess cell viability non-destructively over periods extending up to a few weeks.
The cytotoxicity of hollow gold nanoshells on HAECs was evaluated by the MTT assay (3- 4,5-dimethyl-2-thiazolyl -2,5-diphenyl-2-H-tetrazolium bromide), which is a simple nonradioactive colorimetric assay to assess cell viability and cytotoxicity.
In this work, we demonstrate that optical coherence microscopy (OCM) can be used to assess cell viability without the addition of exogenous probes in three-dimensional (3D) tissue scaffolds maintained under standard culture conditions.
Floating cells were collected and spotted onto YPD agar to assess cell viability.
AlamarBlue™ (Biosource) was used to assess cell viability according to the protocol supplied.
After the treatment was finished, media and the cell lysates were collected and assay was performed to assess cell viability.
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