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Cells were stained with propidium iodide (Sigma) to assess cell cycle state.
This study was designed to assess cell cycle arrest, the production of nitric oxide (NO) and p53 expression in liriodenine-treated human hepatoma cell lines, including wild-type p53 (Hep G2 and SK-Hep-1).
The DNA content of S2 cells was measured by flow cytometry to assess cell cycle stage following three to five days of SUMO RNAi.
Cell ploidy was used to assess cell cycle status.
We performed parallel experiments to assess cell cycle perturbation when gemcitabine was combined with MK-8776.
BrdU incorporation and flow cytometry analysis were used to assess cell cycle progression.
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It applies similar principles as FACS in assessing cell cycle distribution where the intensity of Hoechst stained nucleus is deemed proportional to the cell's DNA content.
We verified the rapamycin resistance of this KSHV+EBV- primary effusion lymphoma cell line, BCBL-1 [23], to growth arrest by treating with the drug and assessing cell cycle profiles.
In addition, we assessed cell cycle progression using flow cytometry with propidium iodide staining of cellular DNA content.
To determine whether the BST2 expression contributes to cell proliferation and apoptosis profiles, we assessed cell cycle and apoptosis profiles by FACS in a recombinant BST2 cell model.
To better investigate the effect of GPI on FLS proliferation, we assessed cell cycle progression, and found that G1-S transition was blocked after GPI knockdown.
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