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T. castaneum consensus genome maps aligned to 124.04 Mb of the in silico maps created from the Tcas5.0 assembly validating 81%% of the draft sequence assembly (Table 3 and Fig. 5).
The rainbow trout physical map can be searched and viewed online via WebFPC: The physical map assembly was validated by: 1) comparing contigs assembly to the probe hybridization results and agarose gel fingerprinting contigs of Palti et al. [ 5]; and 2) anchoring large contigs to the microsatellite-based genetic linkage map of Rexroad et al. [ 15].
The de novo genome assembly was validated by mapping raw reads to the assembled genome.
Reads on paired-end constructs were assembled using GS De Novo Assembler and gaps closed by primer walking; assembly was validated by PFGE.
The control effect of the drive assembly is validated by ground tests.
Numerical examples of a three degree-of-freedom mass-spring system under different conditions and GARTEUR assembly structure validated the feasibility and effectiveness of the proposed method.
After extracting 369,022 cp sequencing reads from our whole-genome-shotgun data, we put together an assembly and validated it with intensive PCR-based verification, coupled with PCR product sequencing.
The physical map assembly was validated at several scales.
The assembly was validated using paired-end information on the Burrows-Wheeler Aligner 0.5.9 (BWA) [ 50].
Correct assembly was validated by reference to a previously described NcoI Optical Map (Opgen) [ 14].
The reliability of the map assembly was validated by PCR assays on randomly selected 21 contigs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com