Exact(1)
Alternative splicing of transcripts and sequence dissimilarity in excess of contig assembly thresholds also contributed to these alignment failures.
Similar(59)
Final assembly threshold for phrap were -minmatch 50 -minscore 100.
I still feel that the case for an assembly threshold and therefore cooperative assembly is weak.
In particular, please consider the point pertaining to cooperative assembly and assembly threshold as mentioned by the reviewer in his/her first point.
However, based on correlation between light scattering and CTP production changes, we predict the assembly threshold of CtpS to be approximately 1 2 μM.
To identify polymorphisms for linkage mapping, contigs from A. mexicanum and A. t. tigrinum assemblies were joined into a single assembly using CAP3 and the following criteria: an assembly threshold of 12 bp to identify initial matches, a minimum 100 bp match length, and 85% sequence identity.
Since this caveat was not clear, we have altered the text to refer to an assembly threshold (a qualitative description of the assembly behavior) rather than a critical concentration, which, as the reviewer notes, is associated with a specific quantitative definition.
The presence of the 2.5-kb plasmid, which had been detected as an extrachromosomal element by agarose gel electrophoresis (data not shown), was not included in the five contigs generated by PacBio, due to its assembly threshold of 7-kb read length.
The kernel program of SWA has eight parameters: the maximum overlapping length max, dynamic overlapping interval L d, read length L r, length of sliding window L w, threshold of repetitive seeds H p, threshold of nonrepetitive seeds L p, threshold of repeats assembly T2, threshold of nonrepeats assembly T1, and sequencing depth after filtering by sliding window Sfd.
And then the parameters are set in advance (see parameters): the maximum overlapping length max, dynamic overlapping interval L d, read length L r, length of sliding window L w, threshold of repetitive seeds H p, threshold of nonrepetitive seeds L p, threshold of repeats assembly T2, threshold of nonrepeats assembly T1, and sequencing depth after filtering by sliding window Sfd.
Over 90% of the reads mapped back to each of the four assemblies, with the most (98%) mapping to the Trinity assembly (Edge threshold = 0.16).
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