The BBC has seen a written submission to the assembly's social development committee in which Ms Palmer said she fully complied with the review, which was completed a year ago, and was assured that she would be given a copy.
The assembly has replaced the original submission, and is available at accession numbers HE605202-HE605210.
Automated gene-calling and functional annotation (EC number, COG, Pfam, TIGRfam, KEGG, Metacyc) was carried out through submission of sequence assembly to Joint Genome Institute's Integrated Microbial Genomes Expert Review (IMG/ER) [ 34].
Annotated coding sequences were evaluated through submission of draft assembly to GenePRIMP annotation improvement platform of the Joint Genome Institute (http://geneprimp.jgi-psf.org) [ 39] to identify long, short, unique, dubious, split or missed genes.
The madness was eventually wrestled into submission, the crib pieces (assembly required) wrestled into a crib.
Submission of a revised assembly and annotations is in progress, and will be accessible under the same project ID.
Following submission of this manuscript, assembly version 7 of the zebrafish genome became available, and all gene predictions were reanalyzed against this assembly.
The DDBJ Pipeline supports not only the use of public domain data, but also the submission of mapping and assembly results to the WGS division of DDBJ in FASTA format.
According to NCBI submission requirement, draft genome assemblies were split into smaller contigs if any sequence contained more than 10 continuous Ns, and the contigs shorter than 200 bp were discarded before the submission to NCBI.
Hot off the assembly line is Western Digital's latest submission into the portable HDD world, the My Passport Elite.
