Exact(8)
The final gaps that could not be closed using the MGView-guided directed PCR procedure (n = 14) corresponded to sequence assembly regions that were unique to strain ET3-1 relatove totherer available SA genome sequences, and were closed by random PCR.
By remapping array results for 40 patients with 1q21 CNVs to the new assembly, regions affected by deletions and duplications were identified with better certainty and precision.
In the reaction mixture, T5 exonuclease degraded the DNA in a 5′ 3′ direction and exposed 3′ overhang in the assembly regions at the ends of each fragment.
We also discarded positions where only a single sequence was found and, in the interspecific analyses, assembly regions where sequences from only one of the species assayed were detected.
Prior to assembly, regions of low-complexity were masked using RepeatBeater (BIOMAX Informatics, Martinsried, Germany) and vector remnants were removed using CrossMatch with the UniVec database [ 87] modified to include the specific polylinkers we used in the cloning process.
The size of the locus varies amongst the hyper-invasive strains with strains 01/04 and 01/35 having the smallest locus of just 14, 746 bp and 01/10 having the largest locus of 35,655 bp, including the flanking transport and assembly regions.
Similar(52)
Figure 2D depicts a 29 cm long and 2 cm wide assembly region with 1524 chips which took 6 minutes to assemble, 2 missing and 4 misaligned components were found in this sample representing a yield of 99.6%.
Inspection of this scaffold showed that the sequence corresponding to the anole protocadherin cluster represents a high-quality assembly region interrupted by 24 gaps.
Each TNC subunit consists of an N-terminal tenascin assembly region, 14.5 epidermal growth factor-like (EGF) repeat domains, a variable number of fibronectin type III-like repeats (FN III) and a C-terminal fibrinogen-like domain [ 12].
The new scaffold is similar to the 138 bp scaffold, but extended on the 3′ end by 51 bp such that the assembly region is now located 58 bases away from the 3′ end.
For instance, the cell-wall assembly protein family Hyr/Iff has been expanded in C. parapsilosis relative to C. orthopsilosis, although the exact number of copies in the latter could not be determined due to the presence of gaps in the relevant assembly region (Riccombeni et al. 2012).
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