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Proper assembly, positioning and orientation of the mitotic spindle are finely orchestrated by molecular players, like the dynein/dynactin complex that ensure correct spindle formation and generate the pulling forces required for proper cell division.
For relatively small genomes, the sequencing of a larger set of individuals could provide enough data to perform de novo sequence assembly, SNP identification and relative sequence assembly positioning.
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The bulk of those jobs, experts were keen to note, were jobs that the United States probably would not want back — like repetitive assembly positions.
Our map shows that this entire 14-MDa Nup82-Nup84 complex assembly positions the cytoplasmic mRNA export factor docking sites and messenger ribonucleoprotein (mRNP) remodeling machinery right over the NPC's central channel rather than on distal cytoplasmic filaments, as previously supposed.
An attempt to outflank the pillboxes failed and the brigade fell back to its assembly positions.
Like or dislike Betsy Butler's political positions is one thing - but it's totally out of the ordinary to have such unrelenting pounding and factually inaccurate savagery directed at a candidate for a simple state assembly position.
During the late hours of 8 April and early morning of 9 April the men of the leading and supporting wave of the attack were moved into their forward assembly positions.
The high sequence coverage generated by NGS for bacterial genomes allows detection of local base differences without the need for whole genome assembly; positions that are consistently different between the mapped reads and the mediator genome are marked as genetic changes (Figure 1).
(EPS 1145 kb) Additional file 3: Distribution of read length and assembly positions.
Instead of recording the closest assembly position for each reference position, we store the closest reference position, U[j] = argmini{| i - j |}, such that there is a match between R[i] and A[j], for each assembly position j.
For this we first fitted a spline of the assembly position on the map position, using the mSNPs.
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