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Thus, the assembly of tangential AJs required Rac activity.
We found that mDia1 and Rac signaling are essential for the assembly of tangential AJs only in nontransformed epithelial cells.
In contrast, myosin II is not required for the assembly of tangential AJs in nontransformed epithelial cells.
Using the RNAi technique for the depletion of mDia1 and loading cells with N17Rac, we established that mDia1 and Rac are involved in the assembly of tangential AJs in nontransformed epithelial cells but not in radial AJs in transformed cells.
While Dia is involved in the assembly of tangential junctions in non-transformed cells, radial junctions are not Dia-dependent in transformed cells.
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Thus, mDia1 contributes to the formation of tangential AJs in nontransformed IAR-2 epithelial cells but is not involved in the assembly and maintenance of radial AJs in transformed epithelial cells and fibroblasts.
In these cells, the deficiency of tangential tension and centripetal tension in the zone of cell-cell contact leads to the assembly of straight actin bundles and to the maturation of radial AJs.
To elucidate the mechanisms of formation of tangential and radial AJs, we next analyzed the involvement of small Rho GTPases and their effectors in AJ assembly.
"I view it as of tangential importance," Emery said.
Are the Arsenal crowd interested in petty booing of tangential links to their neighbours?
Now it is seemingly based on unproven claims of tangential associations with Hamas," Warren said.
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