Our method uses hybridization of DNA strands to form duplexes followed by cation-mediated assembly of quadruplexes.
We have fabricated a DNA-based nanofiber created by self-assembly of guanine quadruplex (Hoogsteen base pairing) and double-stranded DNA (Watson-Crick base pairing).
Nicolas Spinelli (Université Joseph Fourier, France) presented an approach to direct the assembly of oligonucleotides into G-quadruplex architectures (four-stranded structure of stacked guanine tetrads) using a peptide scaffold.
But I think the general public is not interested in the details of quadruplex ligands.
As the Cambridge website notes, this is not the first time that scientists have considered the existence of quadruplex DNA.
Specific guanine-rich patterns can promote formation of quadruplex G-DNA.
Herein, we devise a new signal-enhanced fluorescence aptasensing platform for quantitative screening of lysozyme by coupling with rolling circle amplification (RCA) and strand hybridization reaction, accompanying the assembly of CdTe/CdSe quantum dots (QDs) and hemin/G-quadruplex DNzyme.
Additionally, in order to improve the assembly amount of hemin/G-quadruplex DNAzyme nanowire, the exonuclease-assisted target recycling amplification was used for the continuous removal of S1.
The formation of quadruplexes was followed at 4°C, unless stated otherwise.
There are many things which effect the stability of quadruplexes.
