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De novo assembly of draft genome sequences was achieved from multiplexed Illumina sequence data using Velvet (version 0.7.03) (24), ordered against the S. pneumoniae ATCC 700669 genome using ABACAS (25), and then annotated using Glimmer3 (26).
The advent of second generation sequencing platforms made whole-genome shotgun sequencing of moderately-sized genomes and de novo assembly of draft genome sequences a realistic prospect for non-model organisms.
Furthermore, they are not expected to be well represented in the Bacterial Artificial Chromosome (BAC) libraries used to guide the scaffolding and chromosomal assembly of draft genomes, due to the size-selection of recombinant DNA clones and the natural genomic instability of these regions.
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Further down the assembly line of draft tables, Jennifer Galazin of Amityville was updating Census data on a map of Pennsylvania, and Haimnath Ragbir was working on Port Washington North, comparing a new tax map with the most recent Hagstrom.
Because of the limited size of these scaffolds and the level of assembly of corresponding draft genomes, it is not currently possible to speculate on the nature of the duplication event that has led to 2 genes.
In particular, we found numerous clusters in the macaque genome that seemed to stem from errors in the assembly of the draft genome rather than representing true sequence differences (data not shown).
The assembly of a draft genome and construction of a dense variation catalog are underway.
Data analysis and assembly of the draft manuscript was carried out by KBS, CWW, and KKB.
Data analysis and assembly of the draft manuscript were carried out by SNT, JZ, and KBS.
Data analysis and assembly of the draft manuscript was carried out by KBS, KKB and CWW.
Data analysis and assembly of the draft manuscript was carried out by JZ, SNT, and KBS.
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