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A widely used platform is the 'Golden Gate Cloning', which allows for highly efficient assembly of designed TALEs in a single reaction [ 14, 62, 81, 98 100].
Because the assembly of designed TALE DBDs from nearly identical repeats was challenging for classical cloning techniques, several platforms have emerged for efficient and rapid (less than a week) construction of expression plasmids containing a TALE scaffold with a designed DBD (reviewed in [ 48]).
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Modulated elemental reactants is a method by which new and complex intergrowth compounds can be synthesized by the self-assembly of designed precursors prepared by physical vapor deposition.
However, self-assembly of designed molecules serves as an alternative for the construction of three-dimensional nanoscale systems and is particularly appealing in that material properties can potentially be engineered at the molecular level.
A measurement tool of 41 items that consists of the artifact creativity, design elements and assembly of design elements were utilised for the assessment of 210 artifacts.
Often, the pursuit of Breeam's approval leads buildings to pursue a box-ticking series of technical fixes, and an assembly of products designed to fulfil their requirements without much thought to how they look and feel.
The metal-induced assembly of a designed peptide-based rubredoxin model is described.
Placement and assembly of the designed potabilization plant, is done after the area recognition.
The unifying feature of this approach involves layer-by-layer electrostatic assembly of films designed to detect DNA damage.
Any assembly of devices designed to protect people from hazards or injuries that could arise from the use of the machine can be considered to be a machinery safety system.
That is, selection has on at least two occasions driven the independent assembly of systems designed to produce both beta-lactam antibiotics and overcome beta-lactam resistance.
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