Sentence examples for assembly mix from inspiring English sources

Optimizing the assembly buffer exchange rate, and the rapid introduction of calcium ions to the assembly mix, facilitated the propitious assembly of VLPs.

To synthesize the overlaps necessary for the enzymatic assembly in situ, we added four different oligonucleotides to the enzymatic assembly mix.

After transforming Top 10 E. coli competent cells with 1 μL of the assembly mix, 748 positive colonies were obtained on average.

The assembly mix was incubated at 60 °C for 5 min, 4 °C for 5 min, and finally 50 °C for 60 min. The solution was then dialyzed and electroporated into E. coli BL21 DE3) cells.

Assembly of the full-length DNA construct (20 μL total) was achieved using 35 ng of pMAL vector, 35 ng of the gene of interest, and 15 μL of 1.33× assembly mix.

The assembly mix was then transformed into chemically competent E. coli (T3001, Zymo research, Irvine, CA, USA) according to the manufacturer's instructions, and the gene sequences were confirmed by Sanger sequencing (BaseClear, Leiden, The Netherlands).

Two and three gBlocks were assembled, respectively, using Gibson assembly master mix (New England Biolabs) according to the specification of the manufacturer.

As negative controls, we used a one-step isothermal assembly master mix without Taq ligase and one complete master mix missing the oligonucleotides (Supporting Information Table 4).

Secretion sequences were amplified by PCR and ligated into the SacI site of pDSW206-TALE using Gibson assembly master mix (New England Biolabs).

Phusion DNA polymerase (Finnzymes), Taq DNA ligase (MCLab), and T5 exonuclease (Epicenter) were purchased separately and used to make the assembly master mix (AMM) used for cloning.

The transformations for all other described experiments were done by adding 1 μL of the assembly master mix to Top 10 cells following the instruction of the manufacturer.