Exact(6)
Two and three gBlocks were assembled, respectively, using Gibson assembly master mix (New England Biolabs) according to the specification of the manufacturer.
Phusion DNA polymerase (Finnzymes), Taq DNA ligase (MCLab), and T5 exonuclease (Epicenter) were purchased separately and used to make the assembly master mix (AMM) used for cloning.
As negative controls, we used a one-step isothermal assembly master mix without Taq ligase and one complete master mix missing the oligonucleotides (Supporting Information Table 4).
The transformations for all other described experiments were done by adding 1 μL of the assembly master mix to Top 10 cells following the instruction of the manufacturer.
Secretion sequences were amplified by PCR and ligated into the SacI site of pDSW206-TALE using Gibson assembly master mix (New England Biolabs).
After observing that sequence synthesis from oligonucleotides and DNA assembly can be done simultaneously using a one-step isothermal assembly master mix, we investigated whether the overlaps needed for assembly could be added simultaneously in situ.
Similar(54)
But Chuck Workman's documentary "Magician: The Astonishing Life and Work of Orson Welles" feels almost willfully highlight-intensive, an archival/editing dare for this clip-assembly master to touch on as many elements as possible, as quickly as possible.
James, who had so successfully divided and corrupted Scottish assemblies, never mastered the subtler art of managing an English Parliament.
The master assembly can be accessed in Supporting Information, File S2.
Accordingly, testis tissue had the greatest proportion (ca. 51%) of reads fully mapping to the master assembly (Table 1).
The number of reads per contig was obtained for each tissue separately by mapping its reads of each tissue to the master assembly.
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