The phrase "assembly machineries" is not commonly used in written English and may sound awkward.
It can be used when referring to machines or equipment specifically designed for assembly processes in manufacturing or production contexts.
Example: "The factory invested in new assembly machineries to improve production efficiency."
Alternatives: "assembly equipment" or "assembly machines".
The maturation of mitochondrial Fe-S proteins is carried out via the iron-sulfur cluster (ISC) assembly machinery, whereas cytoplasmic and nuclear Fe-S protein biogenesises depend on both the ISC and CIA (cytosolic iron-sulfur cluster assembly) machineries (Lill et al., 2012; Sipos et al., 2002).
Hence, species-specific structural differences in the CenH3 proteins, such as the long N-terminal tail region that is unique to Drosophila CenH3CID, might result in the use of different assembly machineries.
Briefly, the biosynthetic proteins of both assembly machineries catalyze the condensation of simple building blocks, i.e., carboxylic or amino acids, to produce polymer chains that can be cyclized and decorated to form numerous natural products.
By developing an assay where phospholipid-coated beads were used to track actin assembly machineries at the membrane, we uncovered that Rac1 is indeed insufficient for WRC activation in cell extract; this requires an Arf family GTPase (Koronakis et al., 2011).
Even so, exchange of one of the substituents on the peptidomimetic central fragment 1 to a fluorophore could render attractive compounds for uptake/distribution studies, development of competition-based assays, Förster resonance energy transfer (FRET) studies on binding interactions, and to specifically image conserved pili and curli assembly machineries in bacterial populations.
The different domains of the inner mitochondrial membrane have strikingly different protein contents, e.g., the respiratory chain complexes and supercomplexes are highly enriched in cristae membranes, whereas the mitochondrial import and assembly machineries are preferentially found in the inner boundary membrane (Davies et al., 2012; Vogel et al., 2006).
Böttinger, L. et al. Respiratory chain supercomplexes associate with the cysteine desulfurase complex of the iron-sulfur cluster assembly machinery.
The sorting and assembly machinery (SAM) inserts β-barrel proteins into the mitochondrial outer membrane; it is also known as topogenesis of outer membrane β-barrel proteins (TOB).
The β‐barrel assembly machinery (BAM) complex drives the assembly of β-barrel proteins into the outer membrane of gram-negative bacteria.
The fission yeast Schizosaccharomyces pombe (S. pombe) comprises a small genome featuring organizational elements of more complex eukaryotic systems, including conserved heterochromatin assembly machinery.
Here, we describe approaches we developed to reconstitute the human Fe S cluster assembly machinery in Escherichia coli and to define its remarkable architecture.
