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Before assembly, loci were tested against two quality criteria and loci failing these tests were excluded from the haplotype construction.
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Hence, TAL effector-mediated transcriptional regulation in citrus appears to be further influenced by the multiple protein-protein interactions occurring at the assembly locus of RNA Pol II.
Although there are few in depth analyses on individual bordetellae loci to determine mechanisms or the selective pressures contributing to variation, previous analysis has shown some evidence for HGT in several loci shared by most strains, such as the Pertussis Toxin assembly locus [ 5].
The min2-eye3 linkage on chromosome 2 thus constitutes a second cluster of eyespot-assembly loci in C. reinhardtii in addition to that on chromosome 12.
As suggested in Lamb et al. (1999), the clustering of eyespot-assembly loci may be an instrument for coordinated gene expression that is linked to cell-cycle control.
We first characterized the NCBI m37 C57BL/6J mouse genome assembly Mup loci, within a 1.92 Mb segment of chromosome 4 between Slc46a2 and Zfp37, using a Hidden Markov Model of expressed rodent Mups.
Differences caused by assembly failures (24 loci) or paralogous loci (23 loci pairs) contained cross identified reads (see Additional file 2: Table S2, sections E & F).
This strategy can also be used efficiently for assembly of locus-specific contigs of long-range, highly duplicated regions and targeting all members of a specific gene family, such as MIPS [ 32] and LysM [ 33].
The most parsimonious explanation is therefore that the inferred heterozygosity is an artifact of the assembly of independent loci as a single locus.
This is to be expected from the erroneous assembly of paralogous loci as a single locus, as merged paralogs generate shared polymorphism among populations.
Local hybrid assembly supported 7,708 loci, that is, at least one assembled contig supported the predicted SV at a given locus.
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