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An additional advantage of leaving the poly(A/T) sequence in is that assembled contigs with a poly-A at the end (or poly-T at the start) indicate that the assembly end point is almost certainly correct.
Not only is CTPsyn regulated by multimer assembly, end product inhibition and other allosteric regulators as previously discussed, it is also subject to phosphorylation by multiple kinases (Chang et al., 2007; Choi and Carman, 2007; Kassel et al., 2010).
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The experiments in Figure 4C are controls, and are set up specifically not to show assembly end-points, but rather to ascertain whether the increased abundance of SNARE complexes following disassembly in the presence of Vps33 or Sly1 reflect increases in re-assembly.
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