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Again unsurprisingly, the most significant shared term is "ribosome biogenesis and assembly" (corrected P-value = 5.85e-18) along with other terms related to translation and basic metabolic processes, all processes common among the eukaryotes.
To compare the Pacific Biosciences sequencing to the Illumina de novo assembly, corrected PacBio subreads were aligned to the Illumina A. tequilana assembly using BLAT [ 33] with a minimum threshold of 90% sequence identity.
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Our chromosomal coordinates for the human, the mouse, and the rat, obtained from more recent genome assemblies, correct those reported in two earlier studies (Cho et al. 2005; Cho and Zhang 2006).
Furthermore, this assembly was corrected for mis-assembly errors as demonstrated by resolving a large collapsed repeat and by receiving higher scores by assembly evaluation tools.
For all tests, we call a haplotype assembly correct if it is an exact subsequence of some original COI sequence, otherwise it is incorrect.
This genome assembly has corrected for sequencing errors in the celB sequence currently in GenBank (gi|736356).
Errors in the assembly were corrected using the data from the Illumina sequencing.
The contigs from the assembly were corrected with ∼50 million 76 bp PCR-free paired-end Illumina reads.
Spiral Genetics Anchored Assemblyy performs whole read overlap assembly on corrected, unmapped reads to detect SNVs, indels, and structural variants.
Possible mis-assemblies were corrected with Dupfinisher [25], transposon bombs, and PCR.
During finishing, possible mis-assemblies were corrected by transposon bombing (Epicentre Biotechnologies) of bridging clones.
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