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Sequences reads generated using the Sanger method and by the 454 pyrosequencing were combined and assembled using a hybrid assembly approach developed at the JCVI [18].
In the present study, the draft genome of T. flavidus was sequenced only with the short reads of SOLiD 4 platform and assembled using an assisted assembly approach.
The assembler utilizes a graph-based assembly approach along with individual molecule barcodes to resolve complex repeats and separate chromosomes based on haplotype information for a phased assembly16.
Short reads could be best assembled through de Bruijn Graph (DBG) assembly approach (Miller et al., 2010).
Two new Xiphophorus genomes and their corresponding transcriptomes were efficiently assembled, the former using a novel guided assembly approach.
We sequenced mRNA deriving from this colony using the Illumina sequencing platform and assembled the adult transcriptome of this species de novo using a hybrid assembly approach.
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Our Meta-Assembly approach (Figure 1) consists of four distinct phases: Hybrid Assembly, Scaffold Bridging and Finishing, Scaffold Ordering and Genome Finishing.
Hybrid nanoformulations were prepared through one-step self-assembly approach.
King, P. J. S. et al. A modular self-assembly approach to functionalised β-sheet peptide hydrogel biomaterials.
Hydriodic acid and self-assembly approach are used to fabricate graphene wrapped sulfur composite.
A self-assembly approach is used to fabricate a graphene-coated mesoporous carbon/sulfur composite.
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