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Assemblies were quantified using three measures: 1) the number of contigs containing greater than 100 traces versus the original 35 contigs from individual BAC assemblies 2) average percent coverage of the reassembled contigs to original contigs and 3) average percent nucleotide identity of the reassembled contigs to the original contigs (Table 3).
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Aligned reads were quantified using Cufflinks v1.0.3 transcript assembly and quantification software [ 108].
Proteins were quantified using Qubit® 2.0 Fluorometer.
Immunoblots were quantified using ImageJ.
RNA samples were quantified using spectrophotometry.
Autoradiograms were quantified using ImageJ software.
Immunoblots were quantified using ImageJ software [45].
Phosphorylated proteins were quantified using a densitometer.
Band densities were quantified using Adobe Photoshop.
Signals were quantified using Gene Snap/Gene Tools from SynGene.
PCR products were quantified using ImageJ.
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