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Celera assemblies were assessed with amosvalidate and FRCurve as described [ 37, 38].
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Sequence assembly was assessed with the ContigExpress tool of Vector NTI Software (Invitrogen) using the Nipponbare genomic sequence as reference.
After carrying out several assemblies based on different k-mer lengths, the quality of each assembly was assessed with various parameters and one best assembly was chosen for each isolate (Table 2).
Genome assembly quality and completeness were assessed with multiple benchmark data sets.
The impact of different pore assemblies is assessed for the special case of a bimodal distribution with equal numbers of micro- and macro-pores.
The quality of de novo assemblies was assessed in terms of contiguity and correctness on scaffolds with a size greater than 500 bp.
Completeness of the assemblies was assessed by mapping all reads from each tissue library back to their respective de novo assembly (per species) individually with Bowtie and TopHat [ 46].
Bootstrap values were assessed with 1000 replicates.
The quality of the assembly was assessed by comparing the assembled contigs with a reference set containing well defined mRNA sequences.
Although the accuracy of the detailed hIL-3 receptor α-chain structure may be limited by the modest sequence identity, the overall arrangement of its two domains in the final complex can be assigned with high confidence, so the role of the receptor α-chain in the assembly of the dodecamer complex can be assessed with reasonable certainty (see below).
The completeness of the assembly was assessed by two means, the reconstruction of transcripts with full-length proteins and the representation of core conserved genes.
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