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Assemblies were analysed using RepeatModeler [ 72] to produce repeat libraries that were then combined with known repeats from An. gambiae and retrieved from VectorBase, before being used to mask each genome assembly using RepeatMasker [ 59].
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The 15,858 unique sequences from the global assembly were analysed using two independent methods (ESTScan and a similarity-based approach) to determine the boundaries of the CoDing Sequences (CDS) and UnTranslated Regions (UTR).
For promoter analysis, genomic sequence from the UCSC contig assembly was analysed using the BDGP Neural Network Promoter Prediction program (URL: http://www.fruitfly.org/seq_tools/promoter.html), and the CpG island prediction function of the MethPrimer program (URL: http://itsa.ucsf.edu/~urolab/methprimer) (Li and Dahiya, 2002).
The full sequences of markers were analysed using BLAST http://www.sanger.ac.uk/cgi-bin/blast/submitblast/s_scrofa for identification of clones used in pig genome assemblies.
SO and cyt c were co-assembled in multilayers by alternate adsorption of PASA, and the multilayer assembly was analysed by using QCM, UV Vis spectroscopy and cyclic voltammetry.
All contigs were analysed following the same pipeline that was used to measure the quality of the assemblies.
Four annotated melon scaffolds were analysed for homology with the Cucumis sativus genome assembly deposited in Phytozome v5 [ 50], using the BLASTN algorithm.
All assemblies were generated using Phrap.
Draft assemblies were generated using Newbler 2.6.
Sequence contig assemblies were used as initial source for analyses.
The two global Velvet-Oases assemblies were used in all our subsequent analyses.> -wrap-foot> Total number of distinct protein sequences in best-hit translated BLAST alignments of koala transcripts with Ensembl Tasmanian devil proteins.
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