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We used these data to identify the assemblies of genes uniquely expressed in the germ-line tissues.
We partitioned the network of co-evolutionary relations into clusters and uncovered multiple modular assemblies of genes with clearly defined functions.
Clustering of the co-evolutionary network reveals many examples of modular assemblies of genes that take part in a specific biological process.
However, additional studies are needed to identify which specific assemblies of genes are expressed preferentially within individual tissues and developmental stages.
> This co-evolutionary clustering reveals multiple assemblies of genes that have clear modular functional association (see Supplementary File S3 for the complete list of clusters).
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That is, while genes with low expression levels are more easily assembled (that is longer contigs can be obtained) with small k-mer sizes, assembly of genes with higher expression require large k-mer sizes.
Methods that allow the assembly of genes in one single DNA segment are of great use in bioengineering and synthetic biology.
We present a novel collection of Agrobacterium tumefaciens binary T-DNA vectors compatible with Gateway recombinational cloning that facilitate the modular assembly of genes of interest together with new regulatory sequences, such as strong constitutive or endosperm-specific Brachypodium distachyon promoters.
Pooling the reads allowed the assembly of genes that may only be expressed in one of the two treatments.
Results: We present a novel way of using the de Bruijn graph assembly of metagenomes to improve the assembly of genes.
For all tests that we performed, the application of GeneStitch greatly improves the assembly of genes, resulting in complete or nearly complete genes.
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