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In general, in assemblies of data obtained from sequencing PCR products it is necessary to trim off the primer sequences before assembly in order not to distort the final sequences.
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An initial assembly of data generated from the Illumina GAIIx was performed using Assembly by Short Sequences (ABySS) version 1.2.6 [ 33].
Images from at least 13 metaphase spreads from each data point were quantified before assembly of data in a standard spreadsheet program.
TBA contributed to the conception and design, acquisition and assembly of data, drafting and revision of the manuscript, and final approval of the version to be published.
For this comparison, assembly of data set of genome-paired of FlbB and FlbE proteins was undertaken.
We thank Mia Hall and Alexandra Schmucker for their assistance in annotation of genes and assembly of data in supplementary tables.
Collection and assembly of data: Tiehua Chen, Luming Chen.
Collection and assembly of data: SK, ST.
Collection and assembly of data: PFMK, NT.
Collection and assembly of data: SMB, JAS.
Collection and assembly of data: MafS.
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