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De novo genomic and transcriptomic assemblies generated in this study are shown in bold.
The possible divergence between marine and freshwater medaka at the transcriptome level was assessed by comparisons of sequences deposited in public databases and the assemblies generated in this study.
Gene prediction was undertaken following the MAKER pipeline [ 25] using PST ESTs, de novo transcript assemblies generated in this work (see below), and PGT and P. triticina (PT) peptide sequences as templates for gene model discovery.
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Because de novo assemblies generated in the absence of a reference genome sequence are susceptible to misassembly, we compared our contigs with sequences deposited in GenBank for conifer PAL genes that had been cloned and sequenced in previous studies.
The transcript assembly generated in this study should serve as a useful resource for wheat genomics and genetics.
Compared to the 725 assemblies generated in HMP11,13, this protocol led to improvements in average assembly size, median contig length, and N50 length (Supplementary Table 6).
The morphologies and photophysical properties of the supramolecular assemblies generated in aqueous solutions were evaluated by DLS, TEM, AFM, and steady-state optical spectroscopies.
Finally, a small dataset of 33 WRKY genes and 35 WRKY gene assemblies generated in an experimental study [ 30] gave us an opportunity to directly compare the accuracy of the two gene predictions.
Because neither of the assemblies using 100 or 180 kb as the minimum molecule length improved both assembly metrics when compared to the S t r i c t- T assembly, generated in the first round of selection with the default Minlen of 150 kb, the S t r i c t- T assembly will be referred to as the T. castaneum consensus genome maps in further analysis.
All reads, assemblies, and annotations generated in this study have been deposited in GenBank under BioProject PRJNA257291.
We are in the process of generating an improved reference assembly using the additional sequence generated in this project and other sources in the future.
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