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The Celera assembler did not fully assemble D1-g gene and the fragment varied in length from 30 to 840 nt in different assemblies, whereas the BCM-HGSC assembly positioned the D1-y gene to the 3' side of a D1 gene that was a mix of nucleotides from both D1-g and D1-y.
However, assembly derived from 454 reads plus Illumina reads by using a single assembler did not provide much advantage as compared with the assembly of using only 454 reads.
We assembled sequence data generated by the WGSL and LASL methods using the Assemblez module of ARACHNE with all the default settings except 'recycle_bad_contigs' which was set to 'false' to ensure the assembler did not exclude small, low-coverage contigs.
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"So if the assembler doesn't use fingers, what does it use?" he wrote.
Both the unexpressed pseudogene and its corresponding expressed gene may be reported by the assembler if the implementation of the assembler does not consider such cases.
For metagenomic dataset with uneven abundance ratios, because the IDBA genome assembler does not depend much on coverage to create the de Bruijn graph, the change in abundance ratios will not affect its performance too much.
Other assemblers did not show such an obvious preference.
To our knowledge, other transcriptome sequencing projects using the same assemblers did not report on this phenomenon [ 16- 20], although a large number of unigenes matching to one reference was observed at least once [ 16].
Quality scores are e.g. useful for assembly projects, although some assemblers do not use them.
This was done using the preliminary 4x sequence assembly from the Joint Genome Institute Stanford Human Genome Center, using the Arachne assembler (Batzoglou et al. 2002; Jaffe et al. 2003); later assemblies did not yield additional FPC merges.
But most short read assemblers do not track each individual read back to the assembly [ 11].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com