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GO modules are assembled with the expression values of all its constituting genes.
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Briefly, Cy3 complimentary RNA was labelled with a Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies), and the Cy3-Labeled complimentary RNA was hybridized with the complete genome of S. mutans UA159 assembled with the Agilent Expression Array kit.
One hundred microliters of hybridization solution was dispensed into the gasket slide, which was assembled with the gene expression microarray slide, followed by incubation for 17 hours at 65°C in an Agilent Hybridization Oven.
Genes that were known to have a low expression level were assembled with the same parameter as genes with a very high expression level.
Mapped reads were assembled with the program Cufflinks [ 24] and the expression levels of individual transcripts were calculated as Fragment Per Kilobase of exon per Million fragments mapped (FPKM).
The sequence reads were assembled with the GS Assembler Software (version 2.3).
These were assembled with the Trinity assembler [ 39].
nanoparticles assembled with the protein and aconitine.
The preheater is assembled with the reactor.
Subsequently the sequences were assembled with the known fragments.
Neely assembled with the other first responders.
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