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Once replication is over and the replication checkpoint is turned off, HR centers can be assembled to complete the repair of the accumulated ssDNA gaps and broken ends.
In Escherichia coli, the structure-specific nuclease RuvC serves both of these purposes by processing HR repair intermediates and by mediating RF cleavage so that a new RF may be assembled to complete the replication process.
Sequencing reactions of the target fragments were performed in both directions by 'primer walking' using a Big Dye Terminator Cycle-Sequencing Kit (Applied Biosystems), and overlapping fragments were assembled to complete the sequence of the entire genome.
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The multiple segments obtained by sequencing with different primers were assembled to complete sequences using S EQM AN II™ (DNASTAR®).
Someday I hope to see The New York Times acknowledge that huge groups of supporting workers — managers, accountants, engineers, physicists, chemists and others — assembled and managed to complete the tasks of supporting our astronauts on their journey.
"He came back and said, 'It's too much to take on to do the whole thing, but I want to be involved.' " Dylan contributed one song and assembled other artists to complete the resulting album, titled "The Lost Notebooks of Hank Williams".
Assemble the items needed to complete the project.
Based on the position and sequence information of the assembled contigs, PCR primers were designed to complete the mitogenome sequence of MED (Additional file 2).
The new Give Me 5 program offers a checklist with all the registry's requirements so that small businesses can assemble all the financial data needed to complete the process more smoothly and rapidly.
Assemble the items and tools needed to complete the recipe.
The sequences of the PCR products were determined and assembled to obtain the complete sequence of ahpC, which was deposited in DDBJ with accession number AB819067.
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CEO of Professional Science Editing for Scientists @ prosciediting.com