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Once replication is over and the replication checkpoint is turned off, HR centers can be assembled to complete the repair of the accumulated ssDNA gaps and broken ends.
These sequences were assembled to complete sequences with SeqMan II™ (DNASTAR inc).. Positions where the nucleotide could not be determined with certainty were coded with the appropriate IUPAC code.
In Escherichia coli, the structure-specific nuclease RuvC serves both of these purposes by processing HR repair intermediates and by mediating RF cleavage so that a new RF may be assembled to complete the replication process.
Sequencing reactions of the target fragments were performed in both directions by 'primer walking' using a Big Dye Terminator Cycle-Sequencing Kit (Applied Biosystems), and overlapping fragments were assembled to complete the sequence of the entire genome.
The multiple segments obtained by sequencing with different primers were assembled to complete sequences using S EQM AN II™ (DNASTAR®).
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Afterwards all components have been assembled to a complete fuel processor which has been qualified with various operation parameter set-ups.
Entire fosmids were then sequenced individually and reads equivalent to 20-fold coverage were assembled to generate complete whole contigs.
After domain analyses individual domain results were assembled to a complete protein structure.
Sequenced fragments were assembled to create complete mitochondrial genomes using CodonCode Aligner v3 and BioEdit v7 [ 30].
The sequenced fragments were assembled to create complete mitochondrial genome using CodonCode Aligner v3 and BioEdit v7 [ 32].
Figure 3 presents how mRNA abundances, protein expression and GO terms are assembled to a complete data set and how the resulting biclusters could look like.
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